Z-steps

Russian translation: шаг смещения по оси Z

GLOSSARY ENTRY (DERIVED FROM QUESTION BELOW)
English term or phrase:Z-steps
Russian translation:шаг смещения по оси Z
Entered by: Nitrogen

18:05 Nov 17, 2017
English to Russian translations [PRO]
Science - Biology (-tech,-chem,micro-) / иммунология
English term or phrase: Z-steps
Live imaging of the mouse calvarial BM vasculature of conditioned mice (2 days post-conditioning) or untreated control mice was performed using a custom made multi-photon microscope (Thorlabs, Inc.) incorporating a high pulse energy fiber based femtosecond laser (Cazadero FLCPA, Calmar laser) with excitation wavelengths set at 775 and 950nm. A water-immersed 60x/1.00w objective (LUMPLFLN60XW, Olympus) provided a 415x415 µm field of view and 0.5-5 μm ***Z-steps*** were use to a depth of 150-200 μm.

Спасибо!
Nitrogen
Local time: 02:03
шаг смещения по оси Z
Explanation:
шаг/ шаг z-сканирования / шаг смещения по оси Z / шаг изменения положения объектива / ...
речь идет об увеличении глубины резкости при микроскопии путем т.н. стекинга - сборки последовательных кадров сделанных или с разным фокусным расстоянием или с разного расстояния (последнее предпочтительнее)

см. в книге z-сканирование и z-серии
https://tinyurl.com/yceg5kt7

Пошаговое перемещение плоскости сканирования вдоль оси Z позволяет получить серию контрастных послойных изображений и реконструировать внутреннюю трехмерную структуру (3-D) исследуемого объекта.
http://helpiks.org/7-374.html
Selected response from:

Igor Andreev
Local time: 02:03
Grading comment
Большое спасибо!
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Summary of answers provided
4шаг увеличения
Alexander Onishko
4шаг смещения по оси Z
Igor Andreev
Summary of reference entries provided
z-step
Igor Andreev

  

Answers


14 mins   confidence: Answerer confidence 4/5Answerer confidence 4/5
z-steps
шаг увеличения


Explanation:
наиболее вероятно что Z в данном случае сокращение от zoom

0.5-5 μm ***Z-steps*** were used > использовалось увеличение с шагом 0.5-5 μm

===

interface - How to change the amount of zoom from the scroll wheel ...
https://blender.stackexchange.com/.../how-to-change-the-amou...

Oct 11, 2015 - Ctrl Middle Click is for precise zooming. ... selected (or any vertices, edges or faces selected in Edit Mode) press Z to zoom to that selection

Alexander Onishko
Native speaker of: Native in RussianRussian, Native in UkrainianUkrainian
PRO pts in category: 43
Notes to answerer
Asker: Саша, привет! Хорошая идея. Спасибо

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4 hrs   confidence: Answerer confidence 4/5Answerer confidence 4/5
z-steps
шаг смещения по оси Z


Explanation:
шаг/ шаг z-сканирования / шаг смещения по оси Z / шаг изменения положения объектива / ...
речь идет об увеличении глубины резкости при микроскопии путем т.н. стекинга - сборки последовательных кадров сделанных или с разным фокусным расстоянием или с разного расстояния (последнее предпочтительнее)

см. в книге z-сканирование и z-серии
https://tinyurl.com/yceg5kt7

Пошаговое перемещение плоскости сканирования вдоль оси Z позволяет получить серию контрастных послойных изображений и реконструировать внутреннюю трехмерную структуру (3-D) исследуемого объекта.
http://helpiks.org/7-374.html

Igor Andreev
Local time: 02:03
Specializes in field
Native speaker of: Native in RussianRussian
PRO pts in category: 1427
Grading comment
Большое спасибо!
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Reference comments


4 hrs
Reference: z-step

Reference information:
Nyquist Sampling for Z stacks

If information is to be preserved in all three dimensions, it is important that the images are collected with appropriate x, y and z dimensions. These dimensions are determined by the Nyquist sampling theorem which, in essence, says that, to resolve an object, we must sample that object 2.3 times more frequently than its dimensions. Another way to say this is that our voxel size needs to be a minimum of approximately 0.4 x the dimensions of that object. For example, if the theoretical resolution limit in the lateral (x,y) plane is 0.2μm, then we must use a pixel dimension of 0.2μm x 0.4 = 0.08μm (or 80nm) in that plane. Similarly, if the z dimension resolution limit is 0.5μm, then our best z step should be 0.5μm x 0.4 = 0.2μm (200nm). This means our final microscope setup should be collecting images with voxels that have dimensions of 0.08μm (x) x 0.08μm (y) x 0.2μm(z).


    Reference: http://www.ammrf.org.au/myscope/confocal/confocal/image/zsta...
Igor Andreev
Specializes in field
Native speaker of: Native in RussianRussian
PRO pts in category: 1427
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