sygnał dla kontroli wewnętrznej reakcji
English translation: internal PCR control signal
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| 16:46 Jul 8, 2020 |
| Polish to English translations [PRO] Science - Medical (general) | |||||||
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|  Selected response from: Frank Szmulowicz, Ph. D. United States Local time: 18:10 | ||||||
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| 2 | internal PCR control signal |
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| Discussion entries: 2 | |
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Answers
53 mins confidence: 
internal PCR control signal Explanation: Jedna z sond użytych w tym zestawie jest komplementarna do sekwencji autosomalnej DNA człowieka – sekwencji genu RPPH1 zlokalizowanego na chromosomie 14 o długości amplikonu 140 par zasad. Druga sonda wykrywa selektywnie męskie DNA będąc komplementarną do sekwencji genu SRY o długości 130 par zasad. Dodatkowo w skład zestawu wchodzi kontrola wewnętrzna – IPC (ang. Internal PCR Control) i jest oznaczana za pomocą trzeciej sondy komplementarnej do syntetycznej sekwencji o długości 130 par zasad [4,29]. http://a.umed.pl/zms/dokumenty/2018/P-2-SOBCZAK-PRACA MGR-20... ccc Many real-time quantitative PCR assays like the Plexor® HY System include an internal PCR control (IPC) to test for the presence of PCR inhibitors in the DNA samples. https://www.promega.com/resources/profiles-in-dna/2014/under... ccccc How should it be interpreted if no PCR control signal (HEX) is detected while the target specific signal (FAM) is detected in the positive control? https://www.biocat.com/bc/pdf/TaqMan PCR Kit - General Manua... cccccc In all 22 samples, the PCR control signal was confirmed, and the presence of inhibitors was excluded https://www.researchgate.net/publication/337981190_Compariso... |
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