If you’re fortunate enough to have never experienced this first-hand, you can thank the widespread acceptance of real-time PCR methods, which do away with having to open reaction tubes post amplification, and perhaps gain an appreciation of why anyone who has been through the experience treats the risk as real and ever-present.

At the end of the reaction, and prior to opening any reaction tubes, exposure of the translucent reaction vessels to light causes the (iso)psoralen molecules to cross-link to any DNA present, including amplicon.
https://www.mlo-online.com/home/article/13009432/strategies-...
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Pobieranie kwasu nukleinowego i elucja odbywają się w jednej próbówce w systemie Panther Fusion. Eluat przenoszony jest do próbówki reakcyjnej systemu Panther Fusion, zawierającej odczynniki testu. Następnie przeprowadzany jest test RT-PCR dla wymytego kwasu nukleinowego w systemie Panther Fusion
https://www.hologic.com/sites/default/files/2022-06/AW-25328...