spinner culture

Polish translation: posiew w bioreaktorze z mieszadłem

GLOSSARY ENTRY (DERIVED FROM QUESTION BELOW)
English term or phrase:spinner culture
Polish translation:posiew w bioreaktorze z mieszadłem
Entered by: Frank Szmulowicz, Ph. D.

19:33 Oct 8, 2023
English to Polish translations [PRO]
Science - Biology (-tech,-chem,micro-) / patenty
English term or phrase: spinner culture
Standard infection and expression experiments were done at small scale, using Sf9 insect cells, which were taken from a spinner culture of clean cells that were regularly split to maintain exponential growth
bettys
Poland
Local time: 12:37
posiew w bioreaktorze z mieszadłem
Explanation:
A Spinner is a type of bioreactor which features an impeller, stirrer or similar device to agitate the contents (usually a mixture of cells, medium and products like proteins that can be harvested). The vessels are usually made out of glass or stainless steel with port holes to accommodate sensors, Medium input or gas flow.

Spinner type vessels are used for mammalian or plant cell culture. They are adequate for cell suspensions and attachment dependent cell types.

https://en.wikipedia.org/wiki/Spinner_(cell_culture)



Obecnie głównym nurtem procesu produkcji produktów biologicznych zalecanym przez FDA jest stosowanie kultury zawiesinowej w bioreaktorze z mieszadłem.

http://www.knikbio.com/pl/why-is-the-agitated-bioreactor-ada...

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Note added at 2 hrs (2023-10-08 21:41:57 GMT)
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cccc
Science - Biology (-tech,-chem,micro-)
English term or phrase: spinner flask
mieszalnik
https://www2.proz.com/kudoz/english-to-polish/biology-tech-c...
Selected response from:

Frank Szmulowicz, Ph. D.
United States
Local time: 06:37
Grading comment
3 KudoZ points were awarded for this answer



Summary of answers provided
3posiew w bioreaktorze z mieszadłem
Frank Szmulowicz, Ph. D.
1kultury w zawiesine
geopiet
Summary of reference entries provided
spinner culture of clean cells
geopiet

Discussion entries: 1





  

Answers


49 mins   confidence: Answerer confidence 1/5Answerer confidence 1/5
kultury w zawiesine


Explanation:
A cell suspension or suspension culture is a type of cell culture in which single cells or small aggregates of cells are allowed to function and multiply in an agitated growth medium, thus forming a suspension. Suspension culture is one of the two classical types of cell culture, the other being adherent culture. - https://en.wikipedia.org/wiki/Suspension_culture

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Linie komórkowe mogą rosnąć jako:

kultury adherentne – komórki przylegają do powierzchni naczynia hodowlanego i namnażają się w monowarstwie. Wymagają pasażowania, kiedy hodowla staje się konfluentna, tzn. komórki pokrywają całą powierzchnię naczynia i w coraz większym zagęszczeniu występują zawieszone w pożywce. .

kultury w zawiesine – komórki rosną wolno zawieszone w medium. Kultury takie miesza się przez pipetowanie i odpowiednią ilość pożywki z zawieszonymi komórkami rozcieńcza się w świeżej pożywce. Aby pozbyć się zużytej pożywki, należy odwirować komórki. Po usunięciu supernatantu komórki zawiesza się w świeżej pożywce[8]. Do komórek rosnących w zawiesinie należą komórki hemopoetyczne.

- https://pl.wikipedia.org/wiki/Kultura_komórkowa#Specyfika_ku...

geopiet
Native speaker of: Native in PolishPolish
PRO pts in category: 98
Login to enter a peer comment (or grade)

2 hrs   confidence: Answerer confidence 3/5Answerer confidence 3/5
posiew w bioreaktorze z mieszadłem


Explanation:
A Spinner is a type of bioreactor which features an impeller, stirrer or similar device to agitate the contents (usually a mixture of cells, medium and products like proteins that can be harvested). The vessels are usually made out of glass or stainless steel with port holes to accommodate sensors, Medium input or gas flow.

Spinner type vessels are used for mammalian or plant cell culture. They are adequate for cell suspensions and attachment dependent cell types.

https://en.wikipedia.org/wiki/Spinner_(cell_culture)



Obecnie głównym nurtem procesu produkcji produktów biologicznych zalecanym przez FDA jest stosowanie kultury zawiesinowej w bioreaktorze z mieszadłem.

http://www.knikbio.com/pl/why-is-the-agitated-bioreactor-ada...

--------------------------------------------------
Note added at 2 hrs (2023-10-08 21:41:57 GMT)
--------------------------------------------------

cccc
Science - Biology (-tech,-chem,micro-)
English term or phrase: spinner flask
mieszalnik
https://www2.proz.com/kudoz/english-to-polish/biology-tech-c...

Frank Szmulowicz, Ph. D.
United States
Local time: 06:37
Works in field
Native speaker of: Native in EnglishEnglish, Native in PolishPolish
PRO pts in category: 578
Login to enter a peer comment (or grade)




Reference comments


43 mins
Reference: spinner culture of clean cells

Reference information:
2.1 INSECT CELL CULTURES

Standard infection and expression experiments were done at small scale, using Sf9 insect cells, which were taken from a spinner culture of clean cells that were regularly split to maintain exponential growth. - https://patents.google.com/patent/CA3078281A1/en

geopiet
Native speaker of: Native in PolishPolish
PRO pts in category: 98
Login to enter a peer comment (or grade)



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