English to Russian Medical Biology (-tech,-chem,micro-)

The invention also provides a method for obtaining at least two Tn3 scaffolds that bind to at least one or more targets.
This method allows for the screening of agents that act cooperatively to elicit a particular response.
It may be advantageous to use such a screen when an agonistic activity requiring the cooperation of more than one scaffold is required.
This method allows for the screening of cooperative agents without the reformatting of the library to form multimeric complexes.
In one embodiment, the method of the invention comprises contacting a target ligand with a library of the invention under conditions that allow a scaffold:target ligand complex to form, engaging said scaffolds with a crosslinking agent (defined as an agent that brings together, in close proximity, at least two identical or distinct scaffolds) wherein the crosslinking of the scaffolds elicits a detectable response and obtaining from the complex, said scaffolds that bind the target.

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